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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been observed in many tumors, which leads to chromosome instability, and thus may lead to tumorigenesis. This protein was identified to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital role in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already explained. Two further splice variants were being claimed but in depth nucleotide sequence data is not really still offered.
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Blocking peptides are peptides that bind particularly into the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified by the antibody.
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This guideline outlines additional doable optimization of mobile and immunoassay parameters to guarantee the best possible effects are...
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This tutorial outlines additional doable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
Antibodies certain to the blocking peptide not bind into the epitope over the target protein. By comparing the staining from your blocked antibody compared to the antibody by yourself, one can see which staining is certain.
Goal Information and facts Cyclin E belongs for the really conserved cyclin family members, whose customers are characterised by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins show unique expression and degradation designs which contribute into the temporal coordination of every mitotic event. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates within the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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